The study of stress conditions on growth and proteome of Raoultella planticola: a new emerging pathogen

All bacteria can survive and adapt to different stresses, such as fluctuations in temperature, pH oxidative, osmotic pressure occurring their surrounding environments. This study aims evaluate the effects of a variety stress conditions on growth, proteome Raoultella planticola PTCC 1598. R. cells were exposed values temperatures, sodium chloride, pH, hydrogen peroxide stresses. Among conditions, oxidative stress, upon exposure (H2O2) at 4000 ppm concentration was selected for proteomics analysis detail. Approximately, 1400 spots identified two-dimensional gel electrophoresis (2-DE). spots, 85 repeatable using 2D-Platinum software eye confirmation and, nine protein differentially expressed. proteins, six proteins successfully with an MASCOT score greater than 40 (p < 0.05) 2,3-dihydroxybenzoate-2,3-dehydrogenase (oxidoreductase family), hypothetical G787-04832, periplasmic d-galactose-binding protein, uridine phosphorylase (glycosyltransferases), single peptide match cysteine-binding NADP(H) nitroreductase. showed decreased level expression. Based obtained results, we concluded that antiseptic compound could affect cell growth planticola. Therefore, recommend solution containing H2O2 prevent spread new emerging pathogen.